Cho Cell Line Development

Our services in stable cell line development and recombinant protein manufacturing cover the value chain from dna construction and transfection to cho and hek process development to large scale protein production.

Cho cell line development.

Our cgmp compliant facility enables the generation of master cell banks and master working banks mcb mwb. Although there are several options chinese hamster ovary cho cells have emerged as the gold standard expression system for production of therapeutic proteins. Optimizing cho cell line development via targeted control of n glycosylation 3 7 2015 the glycosylation profile of a protein is vital to its success as a biotherapeutic as it affects its efficacy and function and thus has been extensively studied for proteins produced in chinese hamster ovary cells. They have found wide use in studies of genetics toxicity screening nutrition and gene expression particularly to express recombinant proteins.

The development of high yield production cell lines starts with the cloning of the gene of interest into suretech vectors that bear selexis sge selexis genetic elements. Improvements in the sensitivity and. And common derivatives are cho k1 cho dhb11 cho dxb1 cho s cho dg44 etc. Tuesday june 23 eu 1 00 pm cest us 9 00 am pst.

It s in our dna. In our webinar next generation cell line development platform for advanced bispecific antibodies expression we will address the key challenges of expressing bispecifics in chinese hamster ovary cho cells. Development products freedom cho s and dg44 cell line development kits. Creative biolabs is capable of using lisence cgmp cho s or dg44 cells to develop stable cell lines for research and manufacturing use.

All gibco cho cell line development products are cgmp compliant with no need to source components or develop vectors allowing you to focus on moving your workflow from cell line to process development. The process of cell line development starts with introduction of plasmid dna encoding for the gene of interest gene regulatory elements and a selectable marker into the production host cell line a process called transfection a plasmid expression vector is composed of many different elements. They have an unsurpassed capacity to express a variety of proteins with efficiency. These optimally designed dna expression vectors are then transfected into the fully documented selexis sure cho m cell line using the surefection procedure.

Currently most cho cell line development is based on the two systems. Stable cell lines in 5 months at 2 to 10x higher titer than industry standard.